The Department of Biochemistry at the University of Johannesburg in South Africa is seeking to appoint a postdoctoral research fellow in the field of ANALYTICAL BIOCHEMISTRY and PLANT METABOLOMICS. Candidates should ideally have applicable experience in the following techniques: LC-MS, GC-MS and multivariate data analysis.
Available infrastructure includes:
[1] Shimadzu 8030 LC/MS/MS connected to a Shimadzu Nexera ultra-fast UHPLC
[2] Shimadzu LC-IT-TOF-MS enabling LC/MSn high-precision mass analysis
[3] Shimadzu 2010 Ultra GC-MS
[4] Waters UPLC Synapt G1 high definition ion mobility TOF MS
Contact: Prof. Ian Dubery (idubery@uj.ac.za).
Recent completed projects:
[1]. Phytochemistry. 2013, Vol. 94, pp. 84-90. Metabolomic analysis of isonitrosoacetophenone-induced perturbations in phenolic metabolism of Nicotiana tabacum cells. N Madala, P Steenkamp, L Piater, I Dubery.
[2]. PloS ONE. 2014. Vol. 9(1): e87846. doi:10.1371/journal.pone.0087 846.Multi-platform metabolomic analyses of ergosterol-induced dynamic changes in Nicotiana tabacum cells. F Tugizimana, PSteenkamp, L Piater, I Dubery.
[3]. Springer Plus Online. 2014.Vol. 3, pp. 254 Different metabolite distribution patterns in isonitrosoacetophenone-elicited tobacco and sorghum cells as revealed by multivariate statistical models. N Madala, P Steenkamp, L Piater, I Dubery.
[4]. Plant Physiology and Biochemistry. 2014. Vol.84, pp. 87-95.Metabolomic analysis reveals the effect of isonitrosoacetophenone on defense-related pathways in Arabidopsis thaliana. N Madala, PSteenkamp, L Piater, I Dubery.
[5]. Physiological and Molecular Plant Pathology. 2014. Vol. 88, pp. 61-66. Priming agents of plant defence stimulate the accumulation of mono- and di-acylated chlorogenic acids in cultured tobacco cells. M Mhlongo, L Piater, P Steenkamp, N Madala, I Dubery.
[6]. Chemistry Central Journal.2014. Vol. 8, pp 66-. Analyses of chlorogenic acids and related derivatives from Nicotiana tabacum tissues with the aid of UPLC-QTOF-MS/MS based on the in-source collision-induced dissociation method. E Ncube, M Mhlongo, L Piater, P Steenkamp, I Dubery, N Madala.
[7]. Plant Biotechnology Reports. 2015. Vol. 9, pp. 125-136. Metabolomics-derived insights into the manipulation of terpenoid synthesis in Centella asiatica cells by methyl-jasmonate. F Tugizimana, E Ncube, P Steenkamp, I Dubery.
